Preservation Method and Phosphate Buffered Saline Washing Affect the Acute Myeloid Leukemia Proteome

Acute myeloid leukemia (AML) primary cells can be isolated from peripheral blood, suspended with media containing bovine serum and cryoprotectant, and stored in liquid nitrogen before being processed for proteomic analysis by mass spectrometry (MS). The presence of bovine serum and human blood prote...

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Detalhes bibliográficos
Main Authors: Rebecca Wangen, Elise Aasebø, Andrea Trentani, Stein-Ove Døskeland, Øystein Bruserud, Frode Selheim, Maria Hernandez-Valladares
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2018-01-01
Colecção:International Journal of Molecular Sciences
Assuntos:
proteomics
acute myeloid leukemia
preservation
phosphate buffered saline
dimethyl sulfoxide
mass spectrometry
sample preparation
Acesso em linha:http://www.mdpi.com/1422-0067/19/1/296
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