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One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester
<p>Abstract</p> <p>Background</p> <p>Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synt...
Gardado en:
| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BMC
2008-10-01
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| Series: | BMC Biotechnology |
| Acceso en liña: | http://www.biomedcentral.com/1472-6750/8/76 |
| Tags: |
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