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One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

<p>Abstract</p> <p>Background</p> <p>Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synt...

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Main Authors: Merkx Maarten, Meijer EW, van Baal Ingrid, Bastings Maartje MC
Formáid: Artigo
Teanga:Inglês
Foilsithe: BMC 2008-10-01
Sraith:BMC Biotechnology
Rochtain Ar Líne:http://www.biomedcentral.com/1472-6750/8/76
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