Kinetic Characterization of 100 Glycoside Hydrolase Mutants Enables the Discovery of Structural Features Correlated with Kinetic Constants.

The use of computational modeling algorithms to guide the design of novel enzyme catalysts is a rapidly growing field. Force-field based methods have now been used to engineer both enzyme specificity and activity. However, the proportion of designed mutants with the intended function is often less t...

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Egile Nagusiak: Dylan Alexander Carlin, Ryan W Caster, Xiaokang Wang, Stephanie A Betzenderfer, Claire X Chen, Veasna M Duong, Carolina V Ryklansky, Alp Alpekin, Nathan Beaumont, Harshul Kapoor, Nicole Kim, Hosna Mohabbot, Boyu Pang, Rachel Teel, Lillian Whithaus, Ilias Tagkopoulos, Justin B Siegel
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Public Library of Science (PLoS) 2016-01-01
Saila:PLoS ONE
Sarrera elektronikoa:http://europepmc.org/articles/PMC4729467?pdf=render
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