Optimization of a Loop Mediated Isothermal Amplification (LAMP) Assay for In-Field Detection of Dichelobacter nodosus With aprV2 (VDN LAMP) in Victorian Sheep Flocks

Dichelobacter nodosus is the primary etiological agent of footrot in sheep and has a variety of virulence factors. Of these, AprV2, an extracellular protease, has been shown to be capable of causing severe or “virulent” disease symptoms under the right conditions. Due to this, a loop-mediated isothe...

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Main Authors: Nickala Best, Grant Rawlin, Robert Suter, Brendan Rodoni, Travis Beddoe
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019-03-01
Colecção:Frontiers in Veterinary Science
Assuntos:
LAMP
in-field
footrot
ovine
aprV2
on-farm diagnostic
Acesso em linha:https://www.frontiersin.org/article/10.3389/fvets.2019.00067/full
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