Production of a cloned calf from a fetal fibroblast cell line

The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibro...

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Hlavní autoři: Mello M.R.B., Caetano H.V.A., Marques M.G., Padilha M.S., Garcia J.F., Milazzotto M.P., Assumpção M.E.O.A., Lima A.S., Nicácio A.C., Mendes C.M., Oliveira V.P., Visintin J.A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Associação Brasileira de Divulgação Científica 2003-01-01
Edice:Brazilian Journal of Medical and Biological Research
Témata:
Cloning
Nuclear transfer
Bovine
Fetal fibroblasts
Embryo reconstruction
On-line přístup:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100006
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