Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells

Manual analysis of flow cytometry data and subjective gate-border decisions taken by individuals continue to be a source of variation in the assessment of antigen-specific T cells when comparing data across laboratories, and also over time in individual labs. Therefore, strategies to provide automat...

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Bibliografski detalji
Glavni autori: Natasja Wulff Pedersen, P. Anoop Chandran, Yu Qian, Jonathan Rebhahn, Nadia Viborg Petersen, Mathilde Dalsgaard Hoff, Scott White, Alexandra J. Lee, Rick Stanton, Charlotte Halgreen, Kivin Jakobsen, Tim Mosmann, Cécile Gouttefangeas, Cliburn Chan, Richard H. Scheuermann, Sine Reker Hadrup
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2017-07-01
Serija:Frontiers in Immunology
Teme:
major histocompatibility complex multimers
antigen-specific T cells
automated gating
computational analysis
major histocompatibility complex dextramers
flow cytometry
Online pristup:http://journal.frontiersin.org/article/10.3389/fimmu.2017.00858/full
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