Comparison of three commonly used fusion tags for the expression of nanobodies in the cytoplasm of Escherichia coli

Nanobodies (Nbs) are variable domains of the heavy chain of the heavy-chain antibodies (VHHs) found only in camelids and sharks. They have the same binding capability as full-length immunoglobulin G (IgG) but exhibit improved thermal stability and permeability, pointing to potential scientific, medi...

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Principais autores: Ze-hui Liu, Di Huang, Xiang-jing Fu, Peng Cheng, En-qi Du
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis Group 2018-03-01
coleção:Biotechnology & Biotechnological Equipment
Assuntos:
Nanobodies
VHHs
SUMO tag
soluble expression
Acesso em linha:http://dx.doi.org/10.1080/13102818.2017.1420426
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