Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri

Abstract Background Aliarcobacter faecis and Aliarcobacter lanthieri are recently identified as emerging human and animal pathogens. In this paper, we demonstrate the development and optimization of two direct DNA-based quantitative real-time PCR assays using species-specific oligonucleotide primer...

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Main Authors: Mary G. Miltenburg, Michel Cloutier, Emilia Craiovan, David R. Lapen, Graham Wilkes, Edward Topp, Izhar U. H. Khan
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2020-06-01
Colecção:BMC Microbiology
Assuntos:
qPCR
Aliarcobacter faecis
Aliarcobacter lanthieri
Agricultural watershed
Surface water
Assay
Acesso em linha:http://link.springer.com/article/10.1186/s12866-020-01826-3
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