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Standardizing electrophoresis conditions: how to eliminate a major source of error in the comet assay.
In the alkaline comet assay, cells are embedded in agarose, lysed, and then subjected to further processing including electrophoresis at high pH (>13). We observed very large variations of mean comet tail lengths of cell samples from the same population when spread on a glass or plastic substrate...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Frontiers Media S.A.
2015-06-01
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| Colecção: | Frontiers in Genetics |
| Assuntos: | |
| Acesso em linha: | http://journal.frontiersin.org/Journal/10.3389/conf.fgene.2015.01.00048/full |
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