Cloning, expression and characterization of an aspartate aminotransferase gene from Lactobacillus brevis CGMCC 1306

An aspartate aminotransferase (AATase) gene from Lactobacillus brevis CGMCC 1306 was cloned, which contains a 1182-bp open reading frame coding for 393 amino acids (41.43 kDa). When expressed in Escherichia coli BL21 (DE3), the recombinant AATase was purified and subsequently characterized. The reco...

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Detalhes bibliográficos
Main Authors: Sheng Hu, Xiang Zhang, Yi Lu, Yue-Cheng Lin, Dong-Fang Xie, Hui Fang, Jun Huang, Le-He Mei
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis Group 2017-05-01
Colecção:Biotechnology & Biotechnological Equipment
Assuntos:
Aspartate aminotransferase
psychrophilic enzyme
phase diagram
phylogenetic analysis; Lactobacillus brevis
Acesso em linha:http://dx.doi.org/10.1080/13102818.2017.1304181
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