Library Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes

ABSTRACT Metagenomics is increasingly used to describe microbial communities in biological specimens. Ideally, the steps involved in the processing of the biological specimens should not change the microbiome composition in a way that it could lead to false interpretations of inferred microbial comm...

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Main Authors: Casper S. Poulsen, Claus T. Ekstrøm, Frank M. Aarestrup, Sünje J. Pamp
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2022-04-01
Serier:Microbiology Spectrum
Fag:
microbial communities
metagenomics
library preparation
DNA sequencing
microbiome
metadata
Online adgang:https://journals.asm.org/doi/10.1128/spectrum.00090-22
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