Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
Abstract Background Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, c...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
BMC
2020-03-01
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| Serie: | Microbial Cell Factories |
| Soggetti: | |
| Accesso online: | http://link.springer.com/article/10.1186/s12934-020-01321-4 |
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