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Improved Standardization of Flow Cytometry Diagnostic Screening of Primary Immunodeficiency by Software-Based Automated Gating

BackgroundMultiparameter flow cytometry (FC) is essential in the diagnostic work-up and classification of primary immunodeficiency (PIDs). The EuroFlow PID Orientation tube (PIDOT) allows identification of all main lymphocyte subpopulations in blood. To standardize data analysis, tools for Automated...

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Principais autores: Eleni Linskens, Annieck M. Diks, Jana Neirinck, Martín Perez-Andres, Emilie De Maertelaere, Magdalena A. Berkowska, Tessa Kerre, Mattias Hofmans, Alberto Orfao, Jacques J. M. van Dongen, Filomeen Haerynck, Jan Philippé, Carolien Bonroy
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2020-11-01
Serier:Frontiers in Immunology
Fag:
flow cytometry
immunophenotyping
primary immunodeficiencies
automated gating
standardization
EuroFlow
Online adgang:https://www.frontiersin.org/articles/10.3389/fimmu.2020.584646/full
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