Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis

Previously we used gene-editing to label endogenous EGF receptor (EGFR) with GFP and demonstrate that picomolar concentrations of EGFR ligand drive signaling and endocytosis of EGFR in tumors in vivo (Pinilla-Macua et al., 2017). We now use gene-editing to insert a fluorogen activating protein (FAP)...

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Detalhes bibliográficos

Principais autores:	Mads Breum Larsen, Mireia Perez Verdaguer, Brigitte F Schmidt, Marcel P Bruchez, Simon C Watkins, Alexander Sorkin
Formato:	Artigo
Idioma:	Inglês
Publicado em:	eLife Sciences Publications Ltd 2019-05-01
coleção:	eLife
Assuntos:	EGF receptor endocytosis RNAi
Acesso em linha:	https://elifesciences.org/articles/46135
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