Protocol for affinity enrichment of polyclonal autoantibodies from human plasma

Summary: Autoantibodies (AAbs) contribute to various immune-mediated diseases and are valuable biomarkers for diagnosis, classification, and disease activity. Here, we present a protocol for the affinity enrichment of AAbs from human plasma samples. We describe steps to generate a human cell line ly...

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Autors principals:	Aws Aljnabi, Kelly A. Hagadorn, Louisa E. Neumann, Mary E. Peterson, Bethany Scott, Kazutoyo Miura, Aissata Ongoiba, Safiatou Doumbo, Didier Doumtabe, Shanping Li, Kassoum Kayentao, Boubacar Traore, Silvia Bolland, Peter D. Crompton, Christine S. Hopp
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2025-06-01
Col·lecció:	STAR Protocols
Matèries:	Cell-based Assays Immunology Antibody
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2666166725001364
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