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Improving Hydrolytic Activity and Enantioselectivity of Epoxide Hydrolase from <i>Phanerochaete chrysosporium</i> by Directed Evolution

Epoxide hydrolases (EHs) catalyze the conversion of epoxides into vicinal diols. The epoxide hydrolase gene from <i>P. chrysosporium</i> was previously cloned and subjected to site-directed mutation to study its enzyme activity, but the results were unsatisfactory. This study used error prone PCR an...

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Principais autores: Huanhuan Shao, Pan Xu, Xiang Tao, Xinyi He, Chunyan Pu, Shaorong Liang, Yingxin Shi, Xiaoyan Wang, Hong Feng, Bin Yong
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2024-10-01
Serier:Molecules
Fag:
<i>Phanerochaete chrysosporium</i>
directed evolution
epoxide hydrolases
enantioselectivity
molecular docking
Online adgang:https://www.mdpi.com/1420-3049/29/20/4864
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