An improved CRISPR and CRISPR interference (CRISPRi) toolkit for engineering the model methanogenic archaeon Methanococcus maripaludis

Abstract Background The type II based CRISPR-Cas system remains restrictedly utilized in archaea, a featured domain of life that ranks parallelly with Bacteria and Eukaryotes. Methanococcus maripaludis, known for rapid growth and genetic tractability, serves as an exemplary model for studying archae...

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Bibliografische gegevens

Hoofdauteurs:	Qing Du, Yufei Wei, Liuyang Zhang, Derong Ren, Jian Gao, Xiuzhu Dong, Liping Bai, Jie Li
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	BMC 2024-09-01
Reeks:	Microbial Cell Factories
Onderwerpen:	CRISPR toolbox CRISPR interference Multiplex genome editing Gene expression Archaea Methanogen
Online toegang:	https://doi.org/10.1186/s12934-024-02492-0
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