Solubilization, Activation and Partial Purification of a Sialidase from Horse Liver
Using sialyl-methylumbelliferyl α-glycoside as substrate, sialidase in horse liver was detected as a membrane-bound enzyme. A yield of about 50% of sialidase activity was found in supernatant when solubilized in 0.1 M sodium-phosphate buffer pH 5.5, containing 0.15 M NaCl, 0.25 M sucrose, and 0.5% T...
Guardat en:
| Autors principals: | , , |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Bogor Agricultural University
2005-09-01
|
| Col·lecció: | Hayati Journal of Biosciences |
| Accés en línia: | http://www.sciencedirect.com/science/article/pii/S197830191630331X |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|