Amplification-free long-read sequencing reveals unforeseen CRISPR-Cas9 off-target activity

Abstract Background One ongoing concern about CRISPR-Cas9 genome editing is that unspecific guide RNA (gRNA) binding may induce off-target mutations. However, accurate prediction of CRISPR-Cas9 off-target activity is challenging. Here, we present SMRT-OTS and Nano-OTS, two novel, amplification-free,...

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Detalhes bibliográficos

Principais autores:	Ida Höijer, Josefin Johansson, Sanna Gudmundsson, Chen-Shan Chin, Ignas Bunikis, Susana Häggqvist, Anastasia Emmanouilidou, Maria Wilbe, Marcel den Hoed, Marie-Louise Bondeson, Lars Feuk, Ulf Gyllensten, Adam Ameur
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2020-12-01
coleção:	Genome Biology
Assuntos:	CRISPR-Cas9 On-target Off-target Long-read sequencing Single molecule sequencing PacBio sequencing
Acesso em linha:	https://doi.org/10.1186/s13059-020-02206-w
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