Thermostable in vitro transcription-translation compatible with microfluidic droplets

Abstract Background In vitro expression involves the utilization of the cellular transcription and translation machinery in an acellular context to produce one or more proteins of interest and has found widespread application in synthetic biology and in pharmaceutical biomanufacturing. Most in vitro...

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Principais autores:	Ana L. J. L. Ribeiro, Patricia Pérez-Arnaiz, Mercedes Sánchez-Costa, Lara Pérez, Marcos Almendros, Liisa van Vliet, Fabrice Gielen, Jesmine Lim, Simon Charnock, Florian Hollfelder, J. Eduardo González-Pastor, José Berenguer, Aurelio Hidalgo
Formato:	Artigo
Idioma:	Inglês
Publicado:	BMC 2024-06-01
Series:	Microbial Cell Factories
Assuntos:	In vitro transcription and translation Thermus thermophiles Droplet microfluidics Cell-free protein expression Thermozymes
Acceso en liña:	https://doi.org/10.1186/s12934-024-02440-y
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