Evaluation of DNA extraction kits for long-read shotgun metagenomics using Oxford Nanopore sequencing for rapid taxonomic and antimicrobial resistance detection

Abstract During a bacterial infection or colonization, the detection of antimicrobial resistance (AMR) is critical, but slow due to culture-based approaches for clinical and screening samples. Culture-based phenotypic AMR detection and confirmation require up to 72 hours (h) or even weeks for slow-g...

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Detalhes bibliográficos

Principais autores:	Srinithi Purushothaman, Marco Meola, Tim Roloff, Ashley M. Rooney, Adrian Egli
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Nature Portfolio 2024-11-01
Colecção:	Scientific Reports
Assuntos:	Metagenomics Long-read Taxonomy DNA extraction Protocol ONT
Acesso em linha:	https://doi.org/10.1038/s41598-024-80660-3
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