Fungal Community Profiling and Pathogen Detection in Conifer Seed Lots: Benchmarking Oxford Nanopore DNA Metabarcoding Against Conventional Methods

Seedborne fungal pathogens, either native or exotic, spread through seed trade and pose serious risks to reforestation efforts. Traditional pathogen detection methods, such as seed plating assays, are limited in scope and sensitivity. We assessed the potential of DNA-metabarcoding with Oxford Nanopo...

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Autores principales:	Nicolas Feau, Isabella Laughton, Annie Dicaire, Tod Ramsfield, Philippe Tanguay, Joey B. Tanney
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	The American Phytopathological Society 2025-12-01
Colección:	PhytoFrontiers
Materias:	conifer seeds Fusarium spp. ITS metabarcoding nanopore amplicon sequencing TEF1 metabarcoding translation elongation factor
Acceso en línea:	https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-02-25-0017-R
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