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Immuno-profiling and cellular spatial analysis using five immune oncology multiplex immunofluorescence panels for paraffin tumor tissue

Abstract Multiplex immunofluorescence (mIF) has arisen as an important tool for immuno-profiling tumor tissues. We updated our manual protocol into an automated protocol that allows the use of up to seven markers in five mIF panels to apply to formalin-fixed paraffin-embedded tumor tissues. Using a...

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Principais autores: Edwin Roger Parra, Maria C. Ferrufino-Schmidt, Auriole Tamegnon, Jiexin Zhang, Luisa Solis, Mei Jiang, Heladio Ibarguen, Cara Haymaker, J. Jack Lee, Chantale Bernatchez, Ignacio Ivan Wistuba
Formato: Artigo
Idioma:Inglês
Publicado: Nature Portfolio 2021-04-01
Series:Scientific Reports
Acceso en liña:https://doi.org/10.1038/s41598-021-88156-0
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