Development and Validation of a Next-Generation Sequencing Assay for BRCA1 and BRCA2 Variants for the Clinical Laboratory.

The objective of this study was to design and validate a next-generation sequencing assay (NGS) to detect BRCA1 and BRCA2 mutations. We developed an assay using random shearing of genomic DNA followed by RNA bait tile hybridization and NGS sequencing on both the Illumina MiSeq and Ion Personal Gene...

Descripció completa

Dades bibliogràfiques

Autors principals:	Charles M Strom, Steven Rivera, Christopher Elzinga, Taraneh Angeloni, Sun Hee Rosenthal, Dana Goos-Root, Martin Siaw, Jamie Platt, Cory Braastadt, Linda Cheng, David Ross, Weimin Sun
Format:	Artigo
Idioma:	Inglês
Publicat:	Public Library of Science (PLoS) 2015-01-01
Col·lecció:	PLoS ONE
Accés en línia:	http://europepmc.org/articles/PMC4546651?pdf=render
Etiquetes:	Afegir etiqueta Sense etiquetes, Sigues el primer a etiquetar aquest registre!