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Medium Optimization for Penicillin Acylase (PAc) Production by Recombinant B. megaterium MS941 Containing pac Gene from B. thuringiensis BGSC BD1 Using Response Surface Methodology

Penicillin G acylase (PAc) hydrolyses of the amide bond of benzylpenicillin (Pen-G) releasing PAA and 6-APA, key intermediate in the production of various semisynthetic penicillins. In this study, we optimised the production medium of PAc by RSM using two variables (xylose as inducer and CaCl2 as di...

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Principais autores: FENTRI PARAMITHA PUTRI, ASTUTIATI NURHASANAH, NIKNIK NURHAYATI, IS HELIANTI, KHASWAR SYAMSU
Formato: Artigo
Idioma:Inglês
Publicado em: Indonesian Society for Microbiology 2015-06-01
coleção:Microbiology Indonesia
Assuntos:
Bacillus megaterium btpacBD1
CCD
CaCl2
Penicillin acylase
xylose
Acesso em linha:https://jurnal.permi.or.id/index.php/mionline/article/view/324
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