A simple and rapid technique of template preparation for PCR

Many techniques have been developed for extracting DNA, but most are often complex, time-consuming, and/or expensive. In this study, we describe a simple, rapid and cost-effective technique for preparing DNA template for PCR. This technique involves 0.1 M potassium hydroxide treatment at 100°C for 1...

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Bibliografiske detaljer

Principais autores:	Yunyun Liu, Jia Chen, Yi Cheng, Yi Li, Xinwen Li, Zhengbing Zhang, Xiumei Xu, Yufeng Lin, Jianping Xu, Zhimin Li
Format:	Artigo
Sprog:	Inglês
Udgivet:	Frontiers Media S.A. 2022-11-01
Serier:	Frontiers in Microbiology
Fag:	simple PCR template preparation microorganism potassium hydroxide long fragment amplification high through-put PCR
Online adgang:	https://www.frontiersin.org/articles/10.3389/fmicb.2022.1024827/full
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