High-throughput β-galactosidase and β-glucuronidase Assays Using Fluorogenic Substrates
β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). The protocol described here is a high-throughput alternative to the commonly used Miller assay (Miller, 1972) that utilises a fluorogenic substrate (...
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| Hlavní autor: | |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-protocol LLC
2013-07-01
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| Edice: | Bio-Protocol |
| On-line přístup: | https://bio-protocol.org/en/bpdetail?id=827&type=0 |
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