Probing the Sinorhizobium meliloti-Alfalfa Symbiosis Using Temperature-Sensitive and Impaired-Function Citrate Synthase Mutants

To study the role of the decarboxylating leg of the bacterial TCA cycle in symbiotic nitrogen fixation, we used DNA shuffling and localized random polymerase chain reaction mutagenesis to construct a series of temperature-sensitive and impaired-function mutants in the Sinorhizobium meliloti Rm104A14...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Wojciech Grzemski, James P. Akowski, Michael L. Kahn
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	The American Phytopathological Society 2005-02-01
Schriftenreihe:	Molecular Plant-Microbe Interactions
Schlagworte:	Rhizobium
Online-Zugang:	https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0134
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