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Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection—Protocol Optimization and Pitfalls

Tetrahydrobiopterin (BH4) is an essential cofactor of all nitric oxide synthase isoforms, thus determination of BH4 levels can provide important mechanistic insight into diseases. We established a protocol for high-performance liquid chromatography/electrochemical detection (HPLC/ECD)-based determin...

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Principais autores: Ksenija Vujacic-Mirski, Matthias Oelze, Ivana Kuntic, Marin Kuntic, Sanela Kalinovic, Huige Li, Jacek Zielonka, Thomas Münzel, Andreas Daiber
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2022-06-01
Serier:Antioxidants
Fag:
tetrahydrobiopterin
HPLC with electrochemical detection
oxidative stress
sepsis
Online adgang:https://www.mdpi.com/2076-3921/11/6/1182
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