An improved assay for platelet-activating factor using HPLC-tandem mass spectrometry

We describe an improved assay for platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) using HPLC-tandem mass spectrometry (LC-MS/MS). The present method can readily detect as little as 1 pg (1.9 fmol) of PAF, a significant improvement over previously described LC-MS/MS m...

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Autors principals:	John S. Owen, Robert L. Wykle, Michael P. Samuel, Michael J. Thomas
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2005-02-01
Col·lecció:	Journal of Lipid Research
Matèries:	high-performance liquid chromatography tandem mass spectrometry trace analysis neutrophil
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S0022227520340712
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