Application of Pseudoinfectious Viruses in Transient Gene Expression in Mammalian Cells: Combining Efficient Expression with Regulatory Compliance

Transient gene expression (TGE) is commonly employed for protein production, but its reliance on plasmid transfection makes it challenging to scale up. In this paper, an alternative TGE method is presented, utilizing pseudoinfectious alphavirus as an expression vector. Pseudoinfectious viruses (PIV)...

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Autors principals:	Gulzat Zauatbayeva, Tolganay Kulatay, Bakytkali Ingirbay, Zhanar Shakhmanova, Viktoriya Keyer, Mikhail Zaripov, Maral Zhumabekova, Alexandr V. Shustov
Format:	Artigo
Idioma:	Inglês
Publicat:	MDPI AG 2025-02-01
Col·lecció:	Biomolecules
Matèries:	transient gene expression mammalian expression viral vector alphavirus Venezuelan equine encephalitis virus replicon
Accés en línia:	https://www.mdpi.com/2218-273X/15/2/274
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