CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

Abstract CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an aut...

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Hlavní autoři:	Haiwei Mou, Jordan L. Smith, Lingtao Peng, Hao Yin, Jill Moore, Xiao-Ou Zhang, Chun-Qing Song, Ankur Sheel, Qiongqiong Wu, Deniz M. Ozata, Yingxiang Li, Daniel G. Anderson, Charles P. Emerson, Erik J. Sontheimer, Melissa J. Moore, Zhiping Weng, Wen Xue
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	BMC 2017-06-01
Edice:	Genome Biology
On-line přístup:	http://link.springer.com/article/10.1186/s13059-017-1237-8
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