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Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression in Chlamydomonas reinhardtii via a CRISPR-Cas9-Mediated Knock-in Method

Chlamydomonas reinhardtii is being transformed from a model organism to an industrial organism for the production of pigments, fatty acids, and pharmaceuticals. Genetic modification has been used to increase the economic value of C. reinhardtii. However, low gene-editing efficiency and position-effe...

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Hlavní autoři: Jongrae Kim, Sangmuk Lee, Kwangryul Baek, EonSeon Jin
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2020-03-01
Edice:Frontiers in Plant Science
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On-line přístup:https://www.frontiersin.org/article/10.3389/fpls.2020.00306/full
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