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Truncating the C terminus of formate dehydrogenase leads to improved preference to nicotinamide cytosine dinucleotide

Abstract Formate dehydrogenase (FDH) is widely applied in regeneration of redox cofactors. There are continuing interests to engineer FDH for improved catalytic activity and cofactor preference. In the crystal structure of FDH from Pseudomonas sp. 101 (pseFDH), the C terminus with 9 amino acid resid...

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Principais autores: Xiaojia Guo, Xueying Wang, Yinghan Hu, Lingyun Zhang, Zongbao K. Zhao
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Portfolio 2024-11-01
coleção:Scientific Reports
Assuntos:
Formate dehydrogenase
Directed evolution
Cofactor preference
C terminus
Non-natural cofactor
Acesso em linha:https://doi.org/10.1038/s41598-024-79885-z
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