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Optimisation and Validation of a conventional ELISA and cut-offs for detecting and quantifying anti-SARS-CoV-2 Spike, RBD, and Nucleoprotein IgG, IgM, and IgA antibodies in Uganda

There is an urgent need for better immunoassays to measure antibody responses as part of immune-surveillance activities and to profile immunological responses to emerging SARS-CoV-2 variants. We optimised and validated an in-house conventional ELISA to identify and quantify SARS-CoV-2 spike- (S-), r...

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Hoofdauteurs: Gerald Kevin Oluka, Patricia Namubiru, Laban Kato, Violet Ankunda, Ben Gombe, Matthew Cotten, The COVID-19 Immunoprofiling Team, Monica Musenero, Pontiano Kaleebu, Julie Fox, Jennifer Serwanga, Claire Baine, Jackson Sembera, Susan Mugaba, Christine Hermilia Akoli, Geoffrey Odoch, Deus Mwesigwa, Peter Ejou, Joseph Ssebwana Katende
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Frontiers Media S.A. 2023-03-01
Reeks:Frontiers in Immunology
Onderwerpen:
SARS-CoV-2
spike-protein
Nucleoprotein
RBD
ELISA validation and optimization
antibody seropositivity cut-offs
Online toegang:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1113194/full
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