Protocol for synthesis and mechanical characterization of polyacrylamide hydrogels of varying stiffness for cell culture applications

Summary: The impact of mechanical cues on cell behavior is increasingly being recognized, rendering hydrogel platforms that mimic the extracellular matrix indispensable in in vitro cell biology research. Here, we present a protocol for the synthesis and rheological characterization of polyacrylamide...

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Hauptverfasser:	Charlotte Cresens, Samet Aytekin, Behrad Shaghaghi, Lotte Gerrits, Ana Montero-Calle, Rodrigo Barderas, Paul Kouwer, Susana Rocha
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2026-06-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Cell culture Cell-based Assays High Throughput Screening Molecular Biology Protein expression and purification Biotechnology and bioengineering
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166726002595
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