Protocol for end-design-free rebooting of terminally redundant Pseudomonas phages in clinical isolates of Pseudomonas aeruginosa

Summary: Synthetic phage platforms are robust microbiology tools with therapeutic potential against antimicrobial-resistant bacteria. Here, we present a protocol for rebooting Pseudomonas phages with a terminally redundant, circularly permuted 65 kbp genome. We describe steps for designing PCR prime...

Бүрэн тодорхойлолт

Номзүйн дэлгэрэнгүй

Үндсэн зохиолчид:	Daigo Yokoyama, Nana Kimura, Haruka Yamamoto, Yoshiaki Sakata, Jumpei Fujiki, Hidetomo Iwano
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	Elsevier 2025-09-01
Цуврал:	STAR Protocols
Нөхцлүүд:	Cell culture Microbiology Molecular Biology Biotechnology and bioengineering
Онлайн хандалт:	http://www.sciencedirect.com/science/article/pii/S2666166725004186
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!