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Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis

Abstract Background Lycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene production is by metabolic engineering in microbial hosts, such as Escherichia coli. Isopentenyl diphosphate isomerase (IDI, E.C. 5.3.3.2) is one...

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Principais autores: Hailin Chen, Meijie Li, Changqing Liu, Haibo Zhang, Mo Xian, Huizhou Liu
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2018-04-01
Serier:Microbial Cell Factories
Fag:
Isopentenyl diphosphate isomerase
Random mutagenesis
Enzyme activity
Lycopene production
Mevalonate pathway
Online adgang:http://link.springer.com/article/10.1186/s12934-018-0913-z
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