Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels

Abstract Single-cell RNA sequencing (scRNA-seq) has revolutionized the study of the cellular landscape of organs. Most single-cell protocols require fresh material, which limits sample size per experiment, and consequently, introduces batch effects. This is especially true for samples acquired throu...

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Bibliografski detalji

Glavni autori:	Werna T. C. Uniken Venema, Aarón D. Ramírez-Sánchez, Emilia Bigaeva, Sebo Withoff, Iris Jonkers, Rebecca E. McIntyre, Mennatallah Ghouraba, Tim Raine, Rinse K. Weersma, Lude Franke, Eleonora A. M. Festen, Monique G. P. van der Wijst
Format:	Artigo
Jezik:	Inglês
Izdano:	Nature Portfolio 2022-06-01
Serija:	Scientific Reports
Online pristup:	https://doi.org/10.1038/s41598-022-13812-y
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