An improved primer set and amplification protocol with increased specificity and sensitivity targeting the Symbiodinium ITS2 region

The Internal Transcribed Spacer 2 (ITS2) rRNA gene is a commonly targeted genetic marker to assess diversity of Symbiodinium, a dinoflagellate genus of algal endosymbionts that is pervasively associated with marine invertebrates, and notably reef-building corals. Here we tested three commonly used I...

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Auteurs principaux:	Benjamin C.C. Hume, Maren Ziegler, Julie Poulain, Xavier Pochon, Sarah Romac, Emilie Boissin, Colomban de Vargas, Serge Planes, Patrick Wincker, Christian R. Voolstra
Format:	Artigo
Langue:	Inglês
Publié:	PeerJ Inc. 2018-05-01
Collection:	PeerJ
Sujets:	Coral Reef Symbiodinium Metagenomics Metabarcoding Microbial Biology Next-generation sequencing
Accès en ligne:	https://peerj.com/articles/4816.pdf
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