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Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F m...

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Principais autores: Tshiphiri Senamela, Marleen Kock, Piet Becker, Joachim J.C. Potgieter
Format: Artigo
Sprog:Inglês
Udgivet: AOSIS 2018-01-01
Serier:African Journal of Laboratory Medicine
Fag:
Janus kinase 2
locked nucleic acid
Myeloproliferative neoplasms
Polymerase chain reaction
Polycythaemia vera
Online adgang:https://ajlmonline.org/index.php/ajlm/article/view/658
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