Methodological approaches in aggregate formation and microscopic analysis to assess pseudoislet morphology and cellular interactions [version 2; peer review: 2 approved]

Microscopy has revolutionised our view on biology and has been vital for many discoveries since its invention around 200 years ago. Recent developments in cell biology have led to a strong interest in generating spheroids and organoids that better represent tissue. However, the current challenge fac...

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Bibliographic Details

Main Authors:	Anika Schumacher, Fredrik Wieland, Clemens van Blitterswijk, Nadia Roumans, Timo Rademakers, Vanessa LaPointe
Format:	Artigo
Language:	Inglês
Published:	F1000 Research Ltd 2022-09-01
Series:	Open Research Europe
Subjects:	Three-dimensional cell culture Microwells Immunofluorescence staining 3D Software analysis Cell quantification ROI quantification eng
Online Access:	https://open-research-europe.ec.europa.eu/articles/2-87/v2
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