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Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation.

Propidium monoazide (PMA)-pretreatment has increasingly been applied to remove the bias from dead or damaged cell artefacts, which could impact the microbiota analysis by high-throughput sequencing. Our study aimed to determine whether a PMA-pretreatment coupled with high-throughput sequencing analy...

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Bibliografische Detailangaben
Hauptverfasser: Linh Do Ngoc Nguyen, Pieter Deschaght, Sophie Merlin, Alexandre Loywick, Christophe Audebert, Sabine Van Daele, Eric Viscogliosi, Mario Vaneechoutte, Laurence Delhaes
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science (PLoS) 2016-01-01
Schriftenreihe:PLoS ONE
Online-Zugang:http://europepmc.org/articles/PMC5193350?pdf=render
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