Genome-wide profiling of unmodified DNA using methyltransferase-directed tagging and enrichment

Summary: We present “Active-Seq” (azide click tagging for in vitro epigenomic sequencing), a base-conversion-free technology that enables the isolation of DNA containing unmodified CpG sites using a mutated bacterial methyltransferase enzyme and a synthetically prepared cofactor analog. Active-Seq i...

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Prif Awduron:	Luca Tosti, Calum Mould, Imogen Gatehouse, Anthony C. Smith, Krystian Ubych, Valentina Miano, Peter W. Laird, Jack Kennefick, Robert K. Neely
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Elsevier 2025-10-01
Cyfres:	Cell Reports: Methods
Pynciau:	CP: Molecular biology CP: Genetics
Mynediad Ar-lein:	http://www.sciencedirect.com/science/article/pii/S2667237525002231
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