Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its cytoplasm are to -i- enable disulfide bond formation, -ii- facilitate protein isolation, -iii- control the nature of the N-terminus of the mature protein, and -iv- minimize exposure to cytoplasmic proteases....
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| Principais autores: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Frontiers Media S.A.
2021-12-01
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| coleção: | Frontiers in Bioengineering and Biotechnology |
| Assuntos: | |
| Acesso em linha: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.797334/full |
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