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A novel chimeric avidin with increased thermal stability using DNA shuffling.

Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining random mutagenesis by recombination and selection by a tailored biopanning protoc...

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Autors principals: Barbara Taskinen, Tomi T Airenne, Janne Jänis, Rolle Rahikainen, Mark S Johnson, Markku S Kulomaa, Vesa P Hytönen
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2014-01-01
Col·lecció:PLoS ONE
Accés en línia:http://europepmc.org/articles/PMC3954883?pdf=render
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