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Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues

Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assemb...

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Autori principali: Siou Ku, Claire Heichette, Laurence Duchesne, Denis Chrétien
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2020-08-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=3714&type=0
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