Critique of the pairwise method for estimating qPCR amplification efficiency: beware of correlated data!
Abstract Background A recently proposed method for estimating qPCR amplification efficiency E analyzes fluorescence intensity ratios from pairs of points deemed to lie in the exponential growth region on the amplification curves for all reactions in a dilution series. This method suffers from a seri...
Furkejuvvon:
| Váldodahkki: | |
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| Materiálatiipa: | Artigo |
| Giella: | Inglês |
| Almmustuhtton: |
BMC
2020-07-01
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| Ráidu: | BMC Bioinformatics |
| Fáttát: | |
| Liŋkkat: | http://link.springer.com/article/10.1186/s12859-020-03604-4 |
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