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Elucidating the roles of essential genes in autotrophic metabolism and cell morphology of Clostridium ljungdahlii by CRISPRi

Abstract Understanding the function of essential genes in Clostridium ljungdahlii is critical for unraveling its autotrophic metabolism and optimizing its potential as a platform for syngas fermentation. However, study on essential genes of this species remains insufficient. Here, we employed an ind...

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Main Authors: Saira Munir, Sai Wan, Xinyu Gao, Mingchi Lai, Zhenjie Mu, Hui Wang, Ziyong Liu, Fuli Li, Lin Xia, Yang Tan
Format: Artigo
Language:Inglês
Published: Springer 2026-01-01
Series:Applied Microbiology and Biotechnology
Subjects:
Clostridium ljungdahlii
Syngas fermentation
CRISPR interference
Autotrophic metabolism
Inducible promoters
Essential genes
Online Access:https://doi.org/10.1007/s00253-026-13714-3
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